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EKATERINA M. NESTOROVICH
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Residue Ionization, Selectivity and Ion Transport through OmpF Channel

OmpF channels reconstituted in planar bilayer membranes can endure extreme pH conditions for hours. Exposed to highly acidic or basic environment of pH 1 or pH 12, they can be returned to their completely functional state when solution pH is brought back to neutral. This robustness makes it possible to study channel transport properties over a broad range of solution pH [PDF], [PDF].
 

Changing pH changes ion current through a fully open channel in at least two ways. First, the increase in proton concentration decreases both the average current and the maximum current, measured using 0.1 ms averaging. Second, it changes the character of current fluctuations.

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Collaborators:

"" Tatiana Rostovtseva
"" Vicente M. Aguilella
"" Antonio Alcaraz
 

Changing pH changes ion current through a fully open channel in at least two ways. First, the increase in proton concentration decreases both the average current and the maximum current, measured using 0.1 ms averaging. Second, it changes the character of current fluctuations.

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