Ion Channels as Molecular Coulter Counters
By studying fluctuations in the current mediated by small ions, one can learn not only about the structural changes in the channel protein (e.g., voltage or ligand gating), but also about the transport of larger molecules. Transient occlusions of the channel by these molecules report on their penetration into, and dynamics within, the channel pore.
Related references:
S.M. Bezrukov, O.V. Krasilnikov, L.N. Yuldasheva, A.M. Berezhkovskii, and C.G. Rodrigues. Field-dependent effect of crown ether (18-crown-6) on ionic conductance of alpha-hemolysin channels. Biophysical Journal, 2004, 87:3162-3171. [PDF]
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S.M. Bezrukov, L. Kullman, and M. Winterhalter. Probing sugar translocation through maltoporin at the single channel level. FEBS Letters , 2000, 476:224-228. [PDF]
S.M. Bezrukov. Ion channels as molecular Coulter counters to probe metabolite transport. Journal of Membrane Biology, 2000, 174:1-13. [PDF]
T.K. Rostovtseva and S.M. Bezrukov. ATP transport through a single mitochondrial channel, VDAC, studied by current fluctuation analysis. Biophysical Journal, 1998, 74:2365-2373. [PDF]
S.M. Bezrukov, I. Vodyanoy, R.A. Brutyan, and J.J. Kasianowicz. Dynamics and free energy of polymers partitioning into a nanoscale pore. Macromolecules, 1996, 29:8517-8522. [PDF]
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S.M. Bezrukov and I. Vodyanoy. Probing alamethicin channels with water-soluble polymers. Effect on conductance of channel states. Biophysical Journal, 1993, 64:16-25. [PDF]
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